Environmental Clean up With EPA Methods
• High sample loading ensures effective trace analysis • Simple clean-up procedure saves sample preparation costs • Optimized particle size distribution provides high resolution EnviroPrep columns permit a simple, one stage clean-up to determine pesticides in many organic matrices. The higher molecular weight fractions such as lipids, polymers, natural resins and dispersed high molecular weight components are easily eliminated in the GPC analysis. Preparative GPC for soil extract clean-up is described in “EPA Method 3640A” Method 3640A using 300 x 25 mm and 150 x 25 mm columns to give higher sample loading and fraction yields, which is particularly useful for low levels of pollutants. Low pore size EnviroPrep columns are ideal for this method. The columns have 10 µm particles with 100 Å pore sizes for high resolution, with an exclusion limit of 4000 MW. The preparative columns offer good resolution and high loading through optimization of the particle size distribution.
Columns for sample clean-up
Peak Identification mg/L 1. Corn oil 25,000 2. Bis(2-ethylhexyl) phthalate 1,000 3. Methoxychlor 200 4. Perylene 20 5. Sulfur 80
Microporous Gel for Environmental Clean up
• Self packing loose media for maximum flexibility • Available with glass columns for a complete solution • Use with any LC isocratic system for maximum utility EcoSpheres is a loose microporous media for packing into any suitable column hardware. It operates up to 300 psi and the 25 mm ID glass columns can handle eluent flows up to 10 mL/min. EcoSpheres can be used with any isocratic system, and with a wide range of organic solvents. The narrow particle size distribution gives excellent peak shape and well packed columns, and the low pore size excludes high molecular weight material, maximizing separation from low molecular weight additives.
Separation of trichlorobenzene from sardines
Column: Solvent: Flow Rate: Inj Vol: System: Detector:
Glass column packed with swollen EcoSpheres 50:50 mixture of cyclohexane and ethyl acetate 5 mL/min 5 mL Modular system (dual piston pump with injection valve) UV, 254 nm
Column: Eluent: Flow Rate: Detector:
EnviroPrep, 300 x 25 mm EnviroPrep, 150 x 25 mm DCM 10 mL/min UV, 254 nm
See Also • Polymer Calibration Standards, with highly characterized molecular weights, page 316
EcoSpheres Description Part No. PL1460-4M03 PL1310-0054 PL1310-0055 PL1310-0056 PL1310-0057 PL1310-0058 PL1310-0059
EnviroPrep Columns Description EnviroPrep, 150 x 21.2 mm EnviroPrep, 150 x 25 mm EnviroPrep, 300 x 21.2 mm EnviroPrep, 300 x 25 mm Part No. PL1E10-3120EPA PL1210-3120EPA PL1E10-6120EPA PL1210-6120EPA
EcoSpheres Loose Media (100 g) Complete Glass Column Glass Column Only (no end fittings) Adjustable End Piece Fixed End Piece Seals (Kalrez®) Frits (glass, 10-13 µm)
Visit www.varianinc.com/enviroprep.html or www.varianinc.com/ecospheres.html or visit www.varianinc.com/products.html to place an order.