Bond elut™ Cartridge Spe
Bond Elut C18 OH: Balanced Retention of Polar Analytes
• Silanol activity permits metabolite fractionation • Tight QC tolerances deliver batch-to-batch reproducibility • 150 Å pore size expands retention window to higher molecular weight compounds Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
Bond Elut CBA: Broad Selectivity for Cations
• Cation exchange with no need for extreme basic conditions • Wider selectivity range provides more eluent options • Polar or non-polar depending on matrix or solvent CBA is a mid-polarity sorbent and weak cation exchanger (pKa
4.8). It has a wider range to counter-ions than lower pKa sorbents and easier elution for quaternary amines.
Characteristics Primary retention mechanism Bonded functional group, base material Endcapped Format Weak cation exchange Carboxylic acid Yes Packed bed 7.4 500 40 and 120, irregular 60
Characteristics Primary retention mechanism Bonded functional group, base material Endcapped Format Typical carbon loading (%) Surface area (m /g)
Moderately non-polar Monofunctional octadecyl, acid washed silica No Packed bed 14.9 300 40 and 120, irregular 150
Typical carbon loading (%) Surface area (m2/g) Particle size (µm), shape Mean pore size (Å)
Typical Applications Water, aqueous biological fluids
Particle size (µm), shape Mean pore size (Å)
Typical Applications Water, aqueous biological fluids See also • Bond Elut NEXUS WCX, weak cation exchange polymer, page 40 • Bond Elut SCX, strong cation exchanger, page 49
Bond Elut LRC cartridges (50/box) Sorbent Mass 100 mg 500 mg Volume (mL) 10 10 Part No. 12113011 12113037
See also • Bond Elut C18, universal, non-polar SPE sorbent, page 29 • SPEC™ C18, outstanding flow characteristics, page 21
Bond Elut straight barrel cartridges Sorbent Mass 50 mg 100 mg Quantity (box) 100 50 30 Part No. 12102020 12102046 12256040 200 mg 500 mg 1g 2g Volume (mL) 1 1 3 3 3 6 12 Quantity (box) 100 100 50 50 50 30 20 Part No. 12102073 12102011 12102097 12102124 12102038 12256009 12256058
Sorbent Mass 100 mg 500 mg 1g
Bond Elut straight barrel cartridges Volume (mL) 1 3 6
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