Bond elut™ Cartridge Spe
Bond Elut Mycotoxin: Simplified SPE for Mycotoxin Determination in Food for LC/MS Determination
• Simple methodology saves time and increases throughput • Use with a broad range of food matrices • Economic and time-saving alternative to immunoaffinity techniques Bond Elut Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenone analysis. Results are comparable or superior to competing methods, including immunoaffinity columns (IAC) and charcoal/alumina columns. The sorbent is a proprietary silica-based ion exchange material available in the Bond Elut Jr format. The Bond Elut Mycotoxin method for extraction and clean-up has been successful on a variety of food and grain sample types, including wheat, corn, durum, oats, bread, muesli and infant food. Bond Elut Mycotoxin is easy to use, and acts in a selective non-retention mechanism – the toxin analytes pass through the cartridge while the food matrix components are retained.
References Klötzel, M, Lauber, U & Humpf, H-U (2006) A new solid phase extraction clean-up method for the determination of 12 type A and B trichothecenes in cereals and cereal-based food by LC-MS/MS. Mol. Nutr. Food Res., 50, 261–269. Bretz, M, Beyer, M, Cramer, B & Humpf, H-U (2006) Stable isotope dilution analysis of the fusarium mycotoxins deoxynivalenol and 3-acetyldeoxynivalenol. Mol. Nutr. Food Res., 50, 251-260. Varian Application SI-00295: New SPE sorbent for clean-up of fusarium toxin contaminated cereals and cereal-based foods using BE Mycotoxin. Varian Application SI-01075: Improved isolation and analysis of mycotoxins from cereals, beer and wine- using BE Mycotoxin and Polaris C18-A. Mycotoxin DON ZEA T-2 HT-2 Sake wine % Recovery Mycotoxin DON ZEA T-2 HT-2 94.3 99.3 101.3 113.9 % RSD 7.4 1.3 1.3 8.3 % Recovery 96.8 99.8 66.0 111.0 % RSD 0.5 0.8 0.9 1.0 92.0 116.0 61.3 81.8
Typical Applications Mycotoxins in grain
General mycotoxin methods For Solids 1. Finely grind 25 g sample and extract with a solution of 100 mL acetonitrile/water (80:20) by blending at high speed for 3 min. For simultaneous determination of zearalenone, spike extract at a level of 50 ng/g sample with zearalanone (ZAN) solution in acetonitrile internal standard. Filter. 2. Pass 4 mL of the filtrate through a Bond Elut Mycotoxin column. 3. Evaporate 2 mL of eluate to dryness at 50 °C under a gentle stream of nitrogen. 4. Reconstitute in 0.5 mL ACN/H2O (1:4; v/v). Inject 10 µL into LC for analysis. For Beverages 1. Sonicate the beverage sample for 30 min. Filter. 2. Pass 4 mL of the filtrated sample extract through a Bond Elut Mycotoxin column. 3. Evaporate 2 mL of the eluate to dryness at 50 °C under a gentle stream of nitrogen. 4. Reconstitute in 0.5 mL ACN/H2O (20/80; v/v). 5. Inject into LC/MS/MS. Wheat beer % Recovery % RSD 2.6 6.1 12.6 5.6 % Recovery 95.5 101.9 60.1 76.1 % RSD 1.5 1.3 1.1 1.4
This application shows the optimized extraction and clean up of type A- and B-trichothecenes [deoxynivalenol [DON], HT-2 toxin [HT-2], T-2 toxin [T-2] and zearalenone (ZEA).
Bond Elut Jr (100/box) Sorbent Mass (mg) 500 Bond Elut straight barrel cartridges (50/box) Sorbent Mass (mg) 500 Volume (mL) 3 Part No. 12102167 Part No. 12165001B
See also • Bond Elut Plexa™, advanced polymeric sorbent for bioanalysis, page 12 39
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