Bond elut™ Cartridge Spe
Bond Elut PBA: Covalent Retention Affords Complete Clean up
• Unique sorbent • High specificity for cis-diol compounds • Very effective in separating DNA and RNA Typical Applications Aqueous and biological fluids
PBA is a unique sorbent consisting of a phenylboronic acid covalently linked to a silica gel surface. Covalent processes result in a higher retention to the sorbent surface than other mechanisms, resulting in superior clean-up. The boronate group has a high specificity for cis-diol containing compounds such as catechols, nucleic acids, low molecular weight proteins and carbohydrates. Bond Elut PBA can also be used to retain oligomeric materials such as polyethylene glycols (PEGs) from a variety of aqueous or biological matrices.
GENERIC METHOD Condition: 1. 70:30 H20:ACN with 1% TFA 2. 50mM phosphate buffer (pH 10) Sample Addition: Sample should be buffered to pH 8.5 with 50mM phosphate buffer Interference Wash: 10mM phosphate buffer (pH 8.5) with 5% ACN Analyte Elution: 70:30 H2O:ACN with 1% TFA Compound Class Polyhydroxy Covalent Phenylboronic acid No Packed bed 7.9 500 40, irregular 60
Characteristics Primary retention mechanism Bonded functional group, base material Endcapped Format Typical carbon loading (%) Surface area (m /g) Particle size (µm), shape Mean pore size (Å)
Examples Mannitol, fructose-6phosphate, CDP-ethanol-amine, glycoproteins Catechols, tannins, epinephrine Lactate, 6-phospho-gluconate Salicylate, salicylamide Tris, pyridoxine Dehydroascorbic acid, benzil, alloxan Steroids, prostaglandins
Aromatic O-dihydroxy α-Hydroxy acids Aromatic O-hydroxy acids and amines 1,3-Dihydroxy Diketo & triketo Other dihydroxys
Bond Elut LRC cartridges (50/box) Sorbent Mass (mg) 100 Volume (mL) 10 Part No. 12113018
Bond Elut straight barrel cartridges Sorbent Mass (mg) 100 500 Volume (mL) 1 1 3 Quantity (box) 20 100 50 Part No. 12102018 12102019 12102105
See Also • Bond Elut Plexa™, advanced polymeric sorbent for bioanalysis, page 12 • Bond Elut Plexa PCX, mixed mode polymeric cation exchange, page 13 41
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