pursuit™ upS1.9 C18
Pursuit UPS1.9 C18: Sensitivity and Resolution for Ultra-high Performance Chromatography
• Highest efficiency 1.9 μm particles ensure optimum resolution • Narrow bore columns with minimal band broadening enhance sensitivity • Applicable from discovery to bioanalysis to QA/QC The highly efficient particles and narrow column dimensions of Pursuit UPS1.9 deliver improvements in speed and sensitivity when compared to more traditional HPLC columns. Coupling the UPS silica format with the high carbon load of the Pursuit C18 bonding yields exceptional performance in applications where sensitivity, resolution, and throughput are critical. Pursuit UPS1.9 C18 columns are the first-choice UHPLC solution for difficult separations. Typical Applications Antibiotics
Efficiency comparison of Pursuit columns
Peak Identification 1. Uracil 2. Acetophenone 3. Methylbenzoate 4. Toluene 5. Naphthalene 6. Triphenylene
End capped, Phase; C18 Particle Size (μm) Pore Size (Å) 100 Surface Area (m2/g) 350 Pore Volume (mL/g) 0.9 Carbon Load (%) 21 Ligand Coverage (μmol/m2) 3.0 pH Range 1.5-10
Column: Eluent: Flow rate: Temp:
Various Pursuit C18 Columns, 50 mm x 2.0 mm 60/40 ACN:water 0.4 mL/min 30 °C
Separation of antibiotics with increased efficiency and sensitivity
See Also • Pursuit UPS columns enhance performance with ultra-high pressure, low dead volume chromatographic systems. To achieve similar results on conventional systems, please use our Pursuit Ultra products. See www.varianinc.com for details.
Peak Identification 1. Potassium clavulanate 2. Amoxicillin
ID (mm) 2
Pursuit UPS1.9 C18 High Pressure Packed Fast LC Columns Length (cm) 3 5 10 3 5 10 Part No. A8000030X020H A8000050X020H A8000100X020H A8000050X030H A8000100X030H
A: Pursuit XRs C18 5 µm, 250 x 4.6 mm B: Pursuit UPS1.9 C18, 100 x 2.0 mm Sample Conc: 100 ppm Eluent 20 mM Sodium phosphate buffer pH 4.4:methanol, 95:5 Inj Vol: 1 µL Flow Rate: 0.4 mL/min Temp: 30 °C Detection: UV, 220 nm
Check our Web site for additional chemistries and column dimensions as they become available.
For more information, please visit www.varianinc.com/pursuit_ups.html or visit www.varianinc.com/products.html to place an order.