PL-SAX: Anion Exchange HPLC
• Small particle sizes deliver high efficiency separations • Wide range of particle and column dimensions for productive scale-up • Exceptional stability prolongs column lifetimes PL-SAX is a robust material that delivers separations even under denaturing conditions of elevated temperature, organic solvent, and high pH. It provides improved chromatography for selfcomplimentary or G-rich sequences that may associate to form aggregates or hairpin structures. Excellent chromatographic performance results from small 5 µm particles, for high efficiency separations of n and n-1 sequences. Typical Applications Synthetic peptides and recombinant peptides and proteins
Separation of oligoribonucleotides-oligo(rA)n
Flexibility is delivered by a broad array of particle sizes, 5 µm to 30 µm, and column geometries, 2.1 to 100 mm ID, for analysis and scale-up to purification. The strong anion exchange functionality, quaternary amine, covalently linked to a polymeric particle, provides a material with exceptional chemical and thermal stability, even with sodium hydroxide eluents, leading to long column lifetimes.
Column: Eluent A: Eluent B: Gradient: Flow Rate: Detector: PL-SAX 4000Å 8 µm, 150 x 4.6 mm 20mM KH2PO4 pH 5.5, 5M urea A+1.0M KCl 0-100% B in 200 min 2.0 mL/min UV, 260 nm
Description PL-SAX 1000Å 5 µm PL-SAX 1000Å 8 µm Dimensions (mm) 50 x 2.1 50 x 4.6 50 x 2.1 150 x 2.1 50 x 4.6 150 x 4.6 PL-SAX 4000Å 5 µm PL-SAX 4000Å 8 µm 50 x 2.1 50 x 4.6 50 x 2.1 150 x 2.1 50 x 4.6 150 x 4.6 Part No. PL1951-1502 PL1551-1502 PL1951-1802 PL1951-3802 PL1551-1082 PL1551-3802 PL1951-1503 PL1551-1503 PL1951-1803 PL1951-3803 PL1551-1803 PL1551-3803
See also • PL-SAX, high performance HPLC columns, page 79 79
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