VariTide™ RPC: ‘Universal’ Solution for Analysis and Purification of Synthetic Peptides
• Improved efficiency and resolution give higher purity product in a single run • Long column lifetimes provide more purification cycles per column for improved economics • Fewer columns required, increasing peptide production rate Varian has introduced a single ‘universal’ column for the analysis and purification of synthetic peptides to maximize sample throughput and improve the economics of the purification. This one column replaces a number of columns that would previously have been required for separations of different peptide sizes and eluents, simplifying column choice and minimizing column inventory. VariTide RPC is a high efficiency, small particle packing, with a pore size optimized for high capacity across the peptide size range, from 5 to 60 amino acids. The column is designed to cover the pH range making it suitable for the analysis and purification of acid or basic and hydrophilic or hydrophobic peptides using acid/neutral/ basic eluents.
Column: Eluent A: Eluent B: Gradient: Flow Rate: VariTide RPC 250 x 4.6 mm 0.1% TFA in 95% water : 5% ACN 0.1% TFA in 50% water : 50% ACN 0-100% B in 30 min 1.0 mL/min (360 cm/h)
Typical Applications Peptide analysis and purification at high pH
Crude peptide screen
Description VariTide RPC Columns Dimensions 250 x 4.6 mm 250 x 10 mm 250 x 21.2 mm VariTide Bulk Media 100 g 1 kg Part No. PL1512-5A05 PL1012-5A05 PL1E12-5A05 PL1412-4A05 PL1412-6A05
See also • PLRP-S bulk media, rigid polymeric materials for reverse phase prep to process, page 265 • SepTech™ bulk media, high throughput and high yield purification of synthetic peptides, page 270 81
For more information, please visit www.varianinc.com/varipep.html or visit www.varianinc.com/products.html to place an order.