OMIX™ Tips: For Desalting and Concentrating Peptides and Proteins
• Fast, uniform flow maximizes productivity and reproducibility • Minimal peptide losses lead to higher recoveries • Available in three phases and sizes to deliver better sequence coverage OMIX tips, with monolithic sorbent tip technology, offer dependable purification and superior results in proteomics research. Varian’s OMIX pipette tips reliably purify and enrich femtomole and picomole levels of peptides and proteins prior to MALDI-TOF or LC/MS/MS. The unique monolithic sorbent technology used in OMIX consistently outperforms other tips by delivering uniform flow and strong analyte-to-surface interactions. The high binding capacity of OMIX delivers high productivity; the 10 µL tips bind up to 8 µg of peptide – twice as much as tips from other suppliers. OMIX’s superior flow and exceptional binding capacity ensure reliable recovery of your peptides. OMIX tips minimize peptide losses that occur during multi-aliquot, multi-tip and evaporation steps. The tips come in three sizes; 100 µL, 10 µL, and 10 µL mini-bed to better match your sample volume for downstream MALDI or LC/MS analysis. The OMIX standard 10 µL and mini-bed 10 µL formats can be used for directly spotting desalted proteolytic digests onto a MALDI plate. The OMIX 100 µL format easily purifies larger proteolytic digests that have been fractionated by a first dimensional off-line SCX LC column. The 100 µL format also requires fewer loading cycles, so you can avoid “numb thumb”. OMIX is available in four phases; C4 for protein desalting, C18 for peptide desalting, SCX for detergent removal and MP1 mixed mode cation exchange for basic pharmaceutical compounds. “The most prominent advantage of the OMIX tips over other µC18 tips is a strongly improved flow behavior, which is very important for our viscous samples.” Dr. Josef Wissing, Senior Scientist, Technical University of Braunschweig, Department Biochemistry, Germany.
10 μL C4 C18 SCX Elution volume 2 – 10 μL 1 x 96 tips 6 x 96 tips 1 x 96 tips 6 x 96 tips 1 x 96 tips Part No. A5700910 A5700910K A5700310 A5700310K A5700410 C18 SCX
Typical Applications Protein digestion, peptide extraction, peptide desalting, detergent removal
OMIX for manual pipettors Mini-Bed C4 Elution volume 0.5 – 2 μL 1 x 96 tips 6 x 96 tips 1 x 96 tips 6 x 96 tips 1 x 96 tips Part No. A57009MB A57009MBK A57003MB A57003MBK A57004MB
100 μL C4 C18 SCX
Elution volume 10 – 100μL 1 x 96 tips 6 x 96 tips 1 x 96 tips 6 x 96 tips 1 x 96 tips
Part No. A57009100 A57009100K A57003100 A57003100K A57004100
See also • PL-SAX, strong anion exchange resins, page 250 • PL-SCX, strong cation exchange resins, page 252
For more information, please visit www.varianinc.com/omix_tips.html or visit www.varianinc.com/products.html to place an order.