proteins and large Biomolecules
PLRP-S Wide Pore Columns: For All Sizes of Proteins
• Wide pore sizes deliver high efficiency separations even with large fibrous proteins • Excellent sample recovery and minimal “ghosting” for improved quantification • A gigaporous, 4000Å material, for fast separations Wide pore PLRP-S 300Å, 1000Å and 4000Å HPLC columns for biomolecule analysis are suitable for high performance separations of all sizes of protein. The PLRP-S materials exhibit excellent permeability and provide access for even the largest proteins to the internal surface of the porous particles, giving excellent Typical Applications PLRP-S 300Å globular proteins PLRP-S 1000Å antibodies PLRP-S 4000Å very large proteins, fast separations
Bovine pancreatic zymogens and enzymes
Peak Identification 1. Trypsinogen 2. Trypsin 3. Chymotrypsin 4. Chymotrypsinogen
selectivity and capacity. The superior physical stability, even of the gigaporous 4000Å particles, provides very good column lifetime even with rapid gradients. The chemical stability of the PLRP-S enables column clean-up, even with aggressive protein solubilizing agents, including 1M NaOH, urea and SDS – the limit is the salt solubility in the water/organic eluent.
References Stumpe, M, Miller, C, Morton, NS, Bell, G & Watson, DG (2006) High performance liquid chromatography of a 1-acid glycoprotein in small volumes of plasma from neonates. J. Chromatogr. B, 831, 81-84. Farmery, MR, Tjernberg, LO, Pursglove, SE, Bergman, A, Winblad, B & Näslund, J (2003) Partial purification and characterization of gammasecretase from post-mortem human brain. J. biol. Chem, 278, 2477-2482. Yang, J, Wang, S, Liu, J & Raghani, A (2007) Determination of tryptophan oxidation of monoclonal antibody by reversed phase high performance liquid chromatography. J. Chromatogr. A, 1156, 174-182. Column: Eluent A: Eluent B: Gradient: Flow Rate: Detector: PLRP-S 300Å 8 µm, 150 x 4.6 mm 0.1% TFA in water 0.1% TFA in 95% ACN/5% water 37-40% B in 15 min 40-45% B in 10 min 1.0 mL/min UV, 220 nm
See also • PLRP-S prep to process media, page 265 88 For more information, please visit www.varianinc.com/plrp-s.html or visit www.varianinc.com/products.html to place an order.