proteins and large Biomolecules
PL-SAX, PL-SCX, PL-WAX, PL-WCX: Strong and Weak Ion Exchange Biomolecule Chromatography
• Robust substrate withstands harsh clean-up procedures giving long column lifetimes • High flow rate capability delivers fast separations • Manufactured to ISO 9001:2000 so users can scale-up with confidence Large pore sized polymeric ion exchange resins offer excellent physical and chemical characteristics for the analysis and purification of biomolecules. 1000Å pore sized materials offer maximum capacity for globular proteins whilst gigaporous Typical Applications 1000Å globular proteins, 4000Å high resolution, high throughput
Pepsins in human gastric juice
materials provide a more open structure where high resolution and high throughput are required. The inert polymer substrate provides extended operational column lifetimes by allowing the use of harsh clean-up and depyrogenation procedures to clean and regenerate the column surface.
Sample: Column: Eluent A: Eluent B: Flow Rate: Detector: Courtesy: 250 µL human gastric juice dialyzed against 0.05M sodium acetate, pH 4.1. Filtered 0.45 µm PL-SAX 1000Å 8 µm, 50 x 4.6 mm 50mM Sodium acetate, pH 4.1 A + 1M NaCl 1.0 mL/min UV, 280 nm NB Roberts, Royal Liverpool Hospital, UK.
Dynamic Loading Capacities
Protein BSA Lysozyme Column PL-SAX PL-WAX PL-SCX PL-WCX 1000 Å 100 mg/mL 45 mg/mL 100 mg/mL 65 mg/mL 4000 Å 65 mg/mL 30 mg/mL 25 mg/mL 25 mg/mL
Typing recombinant monoclonal antibody
Column: Eluent A: Eluent B: Gradient: Flow Rate: Detector Courtesy:
PL-SCX 1000Å 8 µm, 150 x 4.6 mm 10mM MES, pH 6.0 0.2M NaCl, 10mM MES, pH 6.0 5% B for 5 min followed by linear 5-85% B over 40 min 1.0 mL/min UV, 280 nm Reprinted from J . Pharma. Biomed. Anal., 16(4), Moorhouse, KG, Nashabeh, W, Deveney, J, Bjork, NS, Mulkerrin, MG & Ryskamp, T, Validation of an HPLC method for the analysis of the charge heterogeneity of the recombinant monoclonal antibody IDEC-C2B8 after papain digestion, 593-603, Copyright 1997 with permission from Elsevier. www.elsevier.com.
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